Sorting and (bio)particle analysis

Welcome to our cell sorting facility!

Based on our own research focus, we offer a strong expertise in both preparative and analytical cell sorting from single cell cloning up to complex immunological investigations.

Customized for your specific research questions, we will assist with choice of device, panel design/optimization and gating strategy, optimization of sample preparation and data analysis.

We are allowed to work under gene safety level S2 and biological safety level S2 (reported bacterial strains) enabling for sorting of patient material and bacterial particles. Extracellular particles/exosomes and lipid droplets are two further examples supported by the high small particle resolution is of our devices.

Below you will find a short description of our devices to give you only a brief impressions of your possibilities.

Please, feel free to contact us via eMail or phone.

We are looking forward to the exciting collaboration with you!

 

                                                                             Contact:

                                                                              Prof. Dr. Daniela Yildiz
                                                                              E-Mail: daniela.yildiz(at)uks.eu
                                                                              fon: 06481/16 26417

 

BD FACSAriaTM Fusion Cell Sorter

Technical specifications:

  • Four-way sorting of at least 25,000 events per second with a purity of >98%
  • Detection of 18 fluorescence parameters
  • Precise, fully automated live control of the drop delay
  • Five high-quality, spatially separated lasers: 355 nm, 405 nm, 488 nm, 561 nm, 640 nm
  • BSL-2 laboratory with a compact microbiological safety cabinet that houses the high-speed cell sorter
  • Can also be used as cell analyser
 

Sony Cell Sorter SH800

Technical specifications:

  • Alignment-free, benchtop sorter with four collinear excitation lasers: 405 nm, 488 nm, 561 nm, 638 nm
  • Microfluidic sorting chip in three sizes (70 μm, 100 μm, 130 μm)
  • Two-way sorter (right, left) in 5 ml or 15 ml tubes
  • Single cell deposition in multiwell microplates / PCR plates
  • Can also be used as cell analyser
  • Operator-free system

 

 

Separation and characterization of macromolecules and nanoparticles

FFF-MALS System is a powerful method for the separation and characterisation of all types of analytes in the range from 1 nm to 1000 nm, such as vectors, viruses and drug carriers,proteins and aggregates.

                        (in procurement)

 

Flow Nanoanalyzer FCM

The Flow NanoAnalyzer can be used for multi-parameter characterisation of natural and synthetic nanoparticles (7-1000 nm) at the single particle level. Such as detection of extracellular vesicles, mitochondria, bacteria, viruses, nanomedicine, nanomaterials and other nanoparticles. By combining light scattering and fluorescence detection, high resolution distributions of particle size and biochemical properties can be obtained simultaneously in 1-2 minutes.

                       (in requesting)