Evaluation of novel drug candidates against multidrug-resistant microbes

Graphical abstract of the wound healing model in SKH-1 mice — © HIPS/IMMHIn vivo evaluation of novel drug candidates in a murine wound infection model.

In cooperation with pharmaceutical sciences at UdS and the Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), IMMH is also addressing the issue of AMR and the need for novel therapeutics by identifying, developing and testing new antimicrobial drug candidates or surface modifications in in vitro and in vivo assays. Susceptibilities of clinical isolates of the ESKAPE (Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterococcus spp.) group of MDR bacteria against drug candidates are tested in classical broth microdilution assays for minimal inhibitory concentrations (MICs). Drug candidates displaying a promising in vitro activity against MDR bacteria and little to no toxicity to human cell cultures are subsequently tested in different murine-based local and systemic infection models.

If you are interested to hear more about this part of IMMH research, please feel free to contact us.

Prof. Dr. Markus Bischoff

Tel.: +49 (0) 6841 16-23963
Fax.: +49 (0) 6841 16-23985
E-Mail: markus.bischoff@uks.eu

Prof. Dr. Dr. Sören Becker

Tel.: +49 (0) 6841 16-23900
Fax: +49 (0) 6841 16-23985
E-Mail: mikrobiologie@uks.eu

Selected publications by members of the research group covering this topic:

Dipeptidic Phosphonates: Potent Inhibitors of Pseudomonas aeruginosa Elastase B Showing Efficacy in a Murine Keratitis Model

Alexander Felix Kiefer, Christian Schütz, Colya Englisch, Dominik Kolling, Samira Speicher, Andreas Martin Kany, Roya Shafiei, Noran Abdel Wadood, Ahmad Aljohmani, Niklas Wirschem, Ravindra Jumde, Andreas Klein, Asfandyar Sikandar, Yu-Mi Park, Gabriela Krasteva-Christ, Daniela Yildiz, Ahmed Saad Abdelsamie, Katharina Rox, Jesko Köhnke, Rolf Müller, Markus Bischoff, Jörg Haupenthal, Anna Katharina Herta Hirsch

ChemRxiv. 2024

DOI: 10.26434/chemrxiv-2024-3mtxb

Establishing an Experimental Pseudomonas aeruginosa Keratitis Model in Mice - Challenges and Solutions.

Colya N. Englisch, Noran Abdel Wadood, Linda Pätzold, Andrew Gallagher, Gabriela Krasteva-Christ, Sören L. Becker, Markus Bischoff (2023)

Ann Anat. 249:152099

DOI: 10.1016/j.aanat.2023.152099

Regio- and Stereoselective Epoxidation and Acidic Epoxide Opening of Antibacterial and Antiplasmodial Chlorotonils Yield Highly Potent Derivatives

Walter Hofer, Emilia Oueis, Antoine Abou Fayad, Felix Deschner, Anastasia Andreas, Laìs Pessanha de Carvalho, Stephan Hüttel, Steffen Bernecker, Linda Pätzold, Bernd Morgenstern, Nestor Zaburannyi, Markus Bischoff, Marc Stadler, Jana Held, Jennifer Herrmann, Rolf Müller (2022)

Angew Chem Int Ed Engl. 61(30):e202202816

DOI: 10.1002/anie.202202816

Evaluation of Bacterial RNA Polymerase Inhibitors in a Staphylococcus aureus-based Wound-Infection Model in SKH1 Mice

Jörg Haupenthal, Yannik Kautz, Walid A M Elgaher, Linda Pätzold, Teresa Röhrig, Matthias W. Laschke, Thomas Tschernig, Anna K. H. Hirsch, Vadim Molodtsov, Katsuhiko S. Murakami, Rolf W. Hartmann, Markus Bischoff (2020)

ACS Infect Dis. 6:2573-2581

DOI: 10.1021/acsinfecdis.0c00034

Functionalization of Chlorotonils: Dehalogenil as Promising Lead Compound for In Vivo Application

Walter Hofer, Felix Deschner, Gwenaelle Jézéquel, Lais Pessanha de Carvalho, Noran Abdel-Wadood, Linde Pätzold, Steffen Bernecker, Bernd Morgenstern, Andreas Martin Kany, Miriam Große, Mark Stadler, Markus Bischoff, Anna Katharina Herta Hirsch, Jana Held, Jennifer Herrmann, Rolf Müller (2024)

Angew Chem Int Ed Engl. 19:e202319765.

DOI: 10.1002/anie.202319765

Efficacy of Off-Label Anti-Amoebic Agents to Suppress Trophozoite Formation of Acanthamoeba spp. on Non-Nutrient Agar Escherichia Coli Plates

Vithusan Muthukumar, Lei Shi, Ning Chai, Achim Langenbucher, Sören L. Becker, Berthold Seitz, Erika Orosz, Tanja Stachon, Albrecht F. Kiderlen, Markus Bischoff, Nóra Szentmáry (2022)

Microorganisms. 2022 Aug 13;10(8):1642

DOI: 10.3390/microorganisms10081642

The effect of antiamoebic agents and Ce6-PDT on Acanthamoeba castellanii trophozoites and cysts, in vitro

Lei Shi, Vithusan Muthukumar, Tanja Stachon, Lorenz Latta, Mohamed Ibrahem Elhawy, Gubesh Gunaratnam, Erika Orosz, Berthold Seitz, Albrecht F. Kiderlen, Markus Bischoff, Nóra Szentmáry (2020)

Transl. Vis. Sci. Technol. 9:29

DOI: 10.1167/tvst.9.12.29

N-Aryl-3-mercaptosuccinimides as antivirulence agents targeting Pseudomonas aeruginosa elastase and Clostridium collagenases

Jelena Konstantinović, Samir Yahiaoui, Alaa Alhayek, Jörg Haupenthal, Esther Schönauer, Anastasia Andreas, Andreas M. Kany, Rolf Müller, Jesko Koehnke, Fabian K. Berger, Markus Bischoff, Rolf W. Hartmann, Hans Brandstetter, Anna K. H. Hirsch (2020)

J Med Chem. 63:8359-8368

DOI: 10.1021/acs.jmedchem.0c00584

Funding sources

Deutsches Zentrum für Infektionsforschung (DZIF)
Marie Skłodowska-Curie Actions (MSCA)
UdS/HIPS Tandem

Logos of sponsors of this research focus: Federal Ministry of Education and Research (BMBF), German Research Foundation (DFG), German Center for Infection Research (DZIF), Clinical Partnerships, Marie Skłodowska-Curie Actions (MSCA) — © IMMH